Copyright 2018 INOE 2000. All rights reserved.
Long Period Grating Fibre Sensor Device for E. Coli Detection
Project acronym: ECOLISENS
Project Coordinator: National Institute of R&D for Optoelectronics INOE 2000 - Romania
Ref.: MNET17/NMCS0042
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Results - Dissemination
With increasing public recognition of threats from bacteria-induced diseases and their potential outbreak among densely populated communities, an intrinsic, low-cost portable biosensor device based on the use of Long Period Grating Fibre Sensor (LPGFS) that can perform quick and precise identification of the E. Coli infection is in high demand to respond to such challenging situations and control the damage those diseases could possibly cause. We can assert that the use of the device will allow to increase the level of sanitary and epidemiologic wellbeing of the population considerably and to prevent such incidents in the future. As are more robust, cheaper, simpler to use and allows proper designs of portable devices compared to SPR sensors, the LPGFS are not dedicated almost exceptional to laboratory use.


  • June 2018 Kick-off meeting took place in Italy
  • 2019 Workshop: ECOLISENS - E. Coli Detection, August 6-8, (Brasov, Romania - Organizer Laurentiu Baschir)
  • August 2019 Project meeting, took place in Romania
  • July 2020 Project Interview - MANUNET - link (Testimonial.pdf)
Transnational Call
2017
Results


Patents request

1. A / 00689/2018 - "Interferometric optoelectronic device in passive optical fiber type TWIN-LPG for the detection of Escherichia coli and Klebsiella pneumoniae bacteria in water from the current network"

2. A / 00234/2020 - "Device with passive optical fiber optoelectronic sensor with a long period of modulation of the refractive index of the core for determining the fluid level in a closed enclosure"



Scientific Papers


1.    Sorin Miclos, Dan Savastru, Roxana Savastru, Ion Lancranjan, "Transverse mechanical stress and optical birefringence induced into singlemode optical fibre embedded in a smart polymer composite material", Comp. Struct., 218, 15-26, 2019.
2.    D. Savastru, S. Miclos, R. Savastru, I. I. Lancranjan, "Analysis of mechanical vibrations applied on a LPGFS smart composite polymer material", Comp. Struct., 226, 111243, 2019.
3.    D. Savastru, S. Miclos, R. Savastru, I. Lancranjan, "Simulation and design of a LPGFS system for detection of Escherichia coli bacteria infestation in milk", J. Optoel. Adv. Mat., 20 (11-12), 610-617, 2018.
4.    S. Miclos, D. Savastru, R. Savastru, I. Lancranjan, "The Simulation and Design of a LPGFS System for detection of Klebsiella in Water", Optoel. Adv. Mat. - Rap. Comm, 13 (1-2), 56-62, 2019.
5.    D. Savastru, S. Miclos, R. Savastru, I. Lancranjan, "Automobile Structural Health Monitoring Performed Using Long Period Grating Fiber Sensors", UPB Sci. Bull. A, 81 (2), 233-244, 2019.
6.    S. Miclos, D. Savastru, R. Savastru, I. Lancranjan, "Torsion Sensor Made of A Super Structure Fiber Bragg Grating Embedded Into Polymer Matrix of an Automobile Composite Material Mechanical Part", UPB Sci. Bull. A, 81 (3), 217-228,  2019.
7.    D. Savastru, S. Miclos, R. Savastru, F. G. Elfarra, I. Lancranjan, "Composite material damage monitoring using embedded in polymer matrix optical fiber bragg grating or long period grating sensors", U.P.B. Sci. Bull., Series A, Vol. 82, Iss. 1, pp.: 253-270, 2020, ISSN 1223-7027, IDS No: KR8AI.
8.    A. Popescu, M. Stafe, D. Savastru, L. Baschir, C. Negutu, N. Puscas, "Study of Surface Plasmon Resonance Structure with As2S3 Amorphous Chalcogenide Compound Waveguide", UPB Sci. Bull. A, 81 (3), 253-264, 2019.
9.    L. Baschir, D. Savastru, A. A. Popescu, I. C. Vasiliu, M. Elisa, I. Chilibon, C. Obreja, "Spectroscopic ellipsometry studies of phosphorus oxide influence on graphene-ZnO sol-gel composite films optical properties", Digest J. Nanomat. Biostruct.
10.   L. Baschir, D. Savastru, A. A. Popescu, I. C. Vasiliu, M. Filipescu, A. M. Iordache, M. Elisa, S. M. Iordache, O. Buiu, C. Obreja, "Morphologic and optical characterization studies of the influence of reduced graphene oxide concentration on optical properties of ZnO-P2O5 composite sol-gel films", Journal of Optoelectronics and Advanced Materials Vol. 21, No. 7-8, July - August, p. 524 - 529, 2019.
11.   Aurelian A. Popescu, Mihai Stafe, Dan Savastru, Laurentiu Baschir, Constantin Negutu and Niculae Puscas, "Study of surface plasmon resonance structure with As2S3 amorphous chalcogenide compound waveguide", U.P.B. Sci. Bull.,      Series A, Vol. 81, Iss. 3, ISSN 1223-7027, 2019.
12.   D. Savastru, R. Savastru, S. Miclos, I. I. Lancranjan, "Simulation of ps laser pulses induced absorption phenomena in materials", Nonconventional Technologies Review, in press.
13.   A. Popescu, D. Savastru, S. Miclos, L. Baschir, M. Tautan, "Non-conventional contact-less method for measurement of materials thermal diffusivity coefficient", Nonconventional Technologies Review, in press.
14.   S. Miclos, L. Baschir, D. Savastru, R. Savastru, I. Lancranjan, "In?ltration in Composite Materials Polymer Matrix Using a Self-Interference Long Period Fiber Sensor", Macromol. Symp. 2020, 389, 1900071, DOI: 10.1002/masy.201900071.
15.   D. Savastru, L. Baschir, S. Miclos, R. Savastru, I. Lancranjan, "Composite Material Cracks Detection Using a Self?Interference Long Period Grating Fiber Sensor", Macromol. Symp. 2020, 389, 1900073, DOI: 10.1002/masy.201900073.
16.   A. A. Popescu, M. Stafe, D. Savastru, L. Baschir, N. Puscas, "Nonlinear Optical Surface Plasmon Resonance in Amorphous Arsenic Sul?de Films", Macromol. Symp., 2020, 389, 1900065,  DOI: 10.1002/masy.201900065.
17.   L. Baschir, A. A. Popescu, D. Savastru, S. Miclos, "Surface Plasmon Resonance Chemical Sensors Based on Amorphous Chalcogenide Waveguides", Macromol. Symp., 2020, 389, 1900066, DOI: 10.1002/masy.201900066.
18.   D. Savastru, S. Miclos, R. Savastru, C. Popa, I. I. Lancranjan, "Simulation/design and development of a long period grating fiber sensor device for pathogen bacteria detection", Acta Materialia Turcica, accepted
19.   A. Popescu, D. Savastru, S. Miclos, L. Baschir, M. Stafe, N. Puscas, "Optical resonance in As2Se3-Au plasmonic planar waveguides for chemical sensors applications", Acta Materialia Turcica, accepted
20.   D. Savastru, L. Baschir, S. Miclos, R. Savastru, I.I. Lancranjan, "Temperature detector based on a SILPG structure embedded into polymermatrix of a smart composite material", Composite Structures, 2020, 245, 112318, pp. 1-7,                             doi:10.1016/j.compstruct. 2020.112318.
21.   A. A. Popescu, D. Savastru, L. Baschir, V. V. Verlan, O. Bordian, M. Stafe, N. Puscas, "Surface plasmon resonance in As2Se3 planar waveguides for the IR spectral region",  Chalcogenide Letters,  Vol. 17, No. 3, 2020, pp.117 -122, IDS            No: LD5JO.
22.   L. Baschir, S. Miclos, D. Savastru, A. A. Popescu, "Surface plasmon resonance using As2S3 film for water salinity detection", Chalcogenide Letters, Vol. 17, No. 1, January 2020, pp.:33-39, IDS No: KL5UH.


Conferences


1.    D. Savastru, S. Miclos, R. Savastru, I. Lancranjan, "The Simulation and Design of a LPGFS System for Detection of Escherichia coli Bacteria in Milk", Joint International Student Conference on Photonics & Modern Laser Applications Conference 2018, ISCP-INDLAS 2018, Alba Iulia, Romania, September 3-7, 2018.
2.    S. Miclos, D. Savastru, R. Savastru, I. Lancranjan, "The Simulation and Design of a LPGFS System for Detection of Klebsiella in Water", Joint International Student Conference on Photonics & Modern Laser Applications Conference 2018, ISCP-INDLAS 2018, Alba Iulia, Romania, September 3-7, 2018.
3.    D. Savastru, S. Miclos, R. Savastru, I. Lancranjan, "Automobile Structural Health Monitoring Performed Using Long Period Grating Fiber Sensors", International Conference of Technology for Polymeric and Composites Products - POLCOM 2018, Bucharest, Romania, 1 November 2018.
4.    S. Miclos, D. Savastru, R. Savastru, I. Lancranjan, "Torsion Sensor Made of A Super Structure Fiber Bragg Grating Embedded Into Polymer Matrix of an Automobile Composite Material Mechanical Part", International Conference of Technology for Polymeric and Composites Products - POLCOM 2018, Bucharest, Romania, 1 November 2018.
5.    D. Savastru, S. Miclos, R. Savastru, I. Lancranjan, "Composite Material Damage Monitoring Using Embedded in Polymer Matrix Optical Fiber Bragg Grating or Long Period Grating Sensors", International Conference of Technology for Polymeric and Composites Products - POLCOM 2018, Bucharest, Romania, 1 November 2018.
6.    S. Miclos, D. Savastru, R. Savastru, I. Lancranjan, "Long Period Grating Fiber Temperature Sensor Embedded Into Polymer Matrix of an Automobile Composite Material Mechanical Part", International Conference of Technology for Polymeric and Composites Products - POLCOM 2018, Bucharest, Romania, 1 November 2018.
7.    A. Popescu, M. Stafe, D. Savastru, L. Baschir, C. Negutu, N. Puscas, "Study of Surface Plasmon Resonance Structure with As2S3 Amorphous Chalcogenide Compound Waveguide" , International Conference of Technology for Polymeric and Composites Products - POLCOM 2018, Bucharest, Romania, 1 November 2018.
8.    D. Savastru, S. Miclos, R. Savastru, I. I. Lancranjan, "Simulation and design of a temperature detector based on a SILPG structure embedded into polymer matrix of a smart composite polymer material", 5th International Conference on Mechanics of Composites, Lisbon, 1 - 4 July 2019.
9.    S. Miclos, D. Savastru, R. Savastru, I. I. Lancranjan, "Detection of impurities using a SILPG embedded into graphene reinforced composites",5th International Conference on Mechanics of Composites, Lisbon, 1 - 4 July 2019.
10.   L. Baschir, A.M. Iordache, D. Savastru, A. A. Popescu, I. C. Vasiliu, M. Elisa, C. Obreja, M. Filipescu, R. Trusca, M. Stchakovsky, "Spectroscopic ellipsometry studies of graphene doped binary TiO2-P2O5 nanocomposite for photocatalytic application", 8th International Conference on Spectroscopic Ellipsometry, May 26-31, Barcelona, Spain, 2019.
11.   Laurentiu Baschir, Sorin Miclos, Dan Savastru and Aurelian A. Popescu, "Surface plasmon resonance using As2S3 film for water salinity", 9th International Conference on Amorphous and Nanostructured Chalcogenides, 30 June-04 July, Chisinau, Republic of Moldova, 2019.
12.   D. Savastru, R. Savastru, S. Miclos, I. I. Lancranjan, "Simulation of ps laser pulses induced absorption phenomena in materials", The 20th International Conference of Nonconventional Technologies, ICNcT 2019, Bucharest, Romania, October 10-11, 2019.
13.   A. Popescu, D. Savastru, S. Miclos, L. Baschir, M. Tautan, "Non-conventional contact-less method for measurement of materials thermal diffusivity coefficient", The 20th International Conference of Nonconventional Technologies, ICNcT 2019, Bucharest, Romania, October 10-11, 2019.
14.   S. Miclos, L. Baschir, D. Savastru, R. Savastru, I. Lancranjan, "Detection of water molecules infiltration in composite materials polymer matrix using a self-interference long period fiber sensor", International Conference on Design and Technologies for Polymeric and Composites Products, POLCOM 2019, Bucharest, Romania, October 10-11, 2019.
15.   D. Savastru, L. Baschir, S. Miclos, R. Savastru, I. Lancranjan, "Composite material cracks detection using a self-interference long period grating fiber sensor", International Conference on Design and Technologies for Polymeric a Composites Products, POLCOM 2019, Bucharest, Romania, October 10-11, 2019.
16.   A. A. Popescu, M. Stafe, D. Savastru, L. Baschir, N. Puscas, "Nonlinear optical surface plasmon resonance in amorphous arsenic sulfide films", International Conference on Design and Technologies for Polymeric and Composites Products, POLCOM 2019, Bucharest, Romania, October 10-11, 2019.
17.   L. Baschir , A. A. Popescu , D. Savastru, S. Miclos, "Surface plasmon resonance chemical sensors based on amorphous chalcodenide waveguides", International Conference on Design and Technologies for Polymeric and Composites Products, POLCOM 2019, Bucharest, Romania, October 10-11, 2019.
18.   D. Savastru, S. Miclos, R. Savastru, C. Popa, I. I. Lancranjan, "Simulation/design and development of a long period grating fiber sensor device for pathogen bacteria detection", 2nd International Conference on Photonics Research - Interphotonics 2019, Kemer, Turkey, November 4-9, 2019.
19.   A. Popescu, D. Savastru, S. Miclos, L. Baschir, M. Stafe, N. Puscas, "Optical resonance in As2Se3-Au plasmonic planar waveguides for chemical sensors applications", 2nd International Conference on Photonics Research - INTERPHOTONICS 2019, Kemer, Turkey, 4-9 Nov. 2019.
20.   S. Miclos, L. Baschir, D. Savastru,  R. Savastru,  I. Lancranjan, "Long period grating fiber sensor for detection of impurities infesting smart polymer composites", 6th International Conference on Mechanics of Composites MECHCOMP6, Porto, Portugal.
21.   D. Savastru, L. Baschir, S. Miclos, R. Savastru,  I. Lancranjan, "Smart composite using fiber optic sensors for fluid flow characterization and temperature measurement", 6th International Conference on Mechanics of Composites MECHCOMP6, Porto, Portugal.
22.   I. Lancranjan, D. Savastru, R. Savastru,  L. Baschir, S. Miclos, "FEM stress analysis of a wind turbine blade made of a smart composite material using diffraction grating optic fiber sensors" , 6th International Conference on Mechanics of Composites MECHCOMP6, Porto, Portugal.
23.   L. Baschir, D. Savastru, S. Miclos, R. Savastru,  I. Lancranjan, "FEM optimization of a smart composite material microphone using a grating fiber optic sensors", 6th International Conference on Mechanics of Composites MECHCOMP6, Porto, Portugal.
24.   R. Savastru,  D. Savastru, L. Baschir, S. Miclos, I. Lancranjan, "Analysis of smart polymer composite delamination using grating optic fiber sensors", 6th International Conference on Mechanics of Composites MECHCOMP6, Porto, Portugal.








SUMMARY

Implementation period: 01/06/2018 - 31/05/2020

The ECOLISENS project aimed to develop a demonstrator for a small, portable and inexpensive device for detecting E. coli bacteria using a long-range fiber optic sensor (LPGFS).
The specific objectives proposed were:
1. Establishing the requirements associated with the use of LPGFS for the detection of E. coli.
2. Determination of the sensor response for changes in the refractive index corresponding to different concentrations of E. coli cells.
3. Determining the operating conditions of the sensor.
4. Defining the basic components of the demonstrator.
5. Completion of the LPGFS query module of the demonstrator.
6. Realization of the data acquisition and processing unit of the demonstrator.
7. Realization of the demonstrator.
8. Demonstration of the capabilities of the sensor device demonstrator.
9. Dissemination of project results.

The objectives were achieved as follows:
1. Establishing the requirements associated with the use of LPGFS for the detection of E. coli was performed in Activity 1.1 (Determining the requirements associated with the use of LPGFS for the detection of E. coli) by developing the E. coli model and demonstrating that the solution to be used simulates in good conditions E. coli.
2. Determining the sensor response for changes in the refractive index corresponding to different concentrations of E. coli cells was performed in Activity 1.2 (Simulations for determining the sensor response for different concentrations of E. coli) by simulating the results obtained at the interrogator, data that were later used to test the demonstrator.
3. The determination of the operating conditions of the sensor was performed in Activity 1.3 (Determining the operating conditions of the sensor) by developing the LPGFS sensor model, using as input data the characteristics of the SMF28e + optical fiber (material, diameters, refractive indices) and by determining LPG characteristics (step and length), which are the basis of the LPGFS sensor.
4. The definition of the basic components of the demonstrator was made within Activity 2.1 (Designing the architecture of the demonstrator) by elaborating the design documentation of the demonstrator. The project was developed for two mounting variants: reflection mounting  and transmission mounting.
5. The demonstrator's LPGFS query module was developed in Activity 3.1 (Development of the Sensor Query Module).
6. The data acquisition and processing unit of the demonstrator was performed within Activity 3.2 (Realization of the Data Acquisition and Processing Unit).
7. The demonstrator was performed in Activity 4.1 (Realization of the demonstrator).
8. The demonstration of the capabilities of the sensor device demonstrator was performed in Activity 5.1 (Demonstrator testing), the demonstrator being tested, and the results were evaluated. The demonstrator was tested with a-Galactosidase solutions of different concentrations, the results showing a sensitivity of the sensor:                                    
9. The dissemination of the project results was carried out within Activities 1.4, 3.3 (Dissemination and working meeting) and within Stages 4 and 5, by creating the project site and the first working meeting of the Consortium and by elaborating scientific articles and participation with papers at scientific conferences;

Estimated results:
1. Establishing the requirements associated with the use of LPGFS for the detection of E. coli.
2. Determining the sensor response for different concentrations of E. coli by simulations.
3. Establishing the operating conditions of the sensor.
4. Demonstrator design documentation.
5. Demonstrator Query Module.
6. Data Acquisition and Processing Unit.
7. Functional demonstrator.
8. Testing and evaluation of test results.
9. Website project.
10. Publication of scientific articles, participation in international conferences and patenting activities.


Obtained results:
1. The requirements associated with the use of LPGFS for the detection of E. coli were established, within Activity 1.1 (Determining the requirements associated with the use of LPGFS for the detection of E. coli).
2. The sensor response for different concentrations of E. coli was determined by simulations, in Activity 1.2 (Simulations for determining the sensor response for different concentrations of E. coli).
3. The operating conditions of the sensor were established, within Activity 1.3 (Determining the operating conditions of the sensor).
4. The design documentation of the demonstrator was elaborated, within Activity 2.1 (Design of the demonstrator architecture).
5. The Demonstrator Interrogation Module was developed, within Activity 3.1 (Realization of the Sensor Interrogation Module).
6. The data acquisition and processing unit of the demonstrator was realized, within Activity 3.2 (Realization of the Data Acquisition and Processing Unit).
7. A functional demonstrator was developed, within Activity 4.1 (Realization of the demonstrator).
8. The demonstrator has been tested.
9. The project website was created: http://ecolisens.inoe.ro/